PROLINE OXIDASES IN HANSENULA SUBPELLICULOSA

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Proline Oxidases in Hansenula Subpelliculosa.

Ling, Chung-Mei (Illinois Institute of Technology, Chicago), and L. R. Hedrick. Proline oxidases in Hansenula subpelliculosa. J. Bacteriol. 87:1462-1470. 1964-Cells of Hansenula subpelliculosa can use l-proline as a carbon and a nitrogen source after a 6- to 8-hr induction period. However, they cannot use l-glutamate as both nitrogen and carbon sources unless the induction period is of several ...

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Kinetic and structural analysis of substrate specificity in two copper amine oxidases from Hansenula polymorpha.

The structural underpinnings of enzyme substrate specificity are investigated in a pair of copper amine oxidases (CAOs) from Hansenula polymorpha (HPAO-1 and HPAO-2). The X-ray crystal structure (to 2.0 A resolution) and steady state kinetic data of the second copper amine oxidase (HPAO-2) are presented for comparison to those of HPAO-1. Despite 34% sequence identity and superimposable active s...

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In vitro dissociation and re-assembly of peroxisomal alcohol oxidases of Hansenula polymorpha and Pichia pastoris.

We have studied the in vitro inactivation/dissociation and subsequent reactivation/re-assembly of peroxisomal alcohol oxidases (AO) from the yeasts Hansenula polymorpha and Pichia pastoris. Both proteins are homo-oligomers consisting of eight identical subunits, each containing one FAD as the prosthetic group. They were both rapidly inactivated upon incubation in 80% glycerol, due to their diss...

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Effect of Cations upon Hydrophobicity of Yeasts Grown in Amino Acid Media.

Hedrick, L. R. (Illinois Institute of Technology, Chicago), and C. J. Feren. Effect of cations upon hydrophobicity of yeasts grown in amino acid media. J. Bacteriol. 86:1288-1294. 1963.-Cells of four species of Hansenula, namely, H. holstii Y2154, H. subpelliculosa Y1683 and Y1542, and H. anomala Y365, were cultured for 3 days in a glucose-salts medium with one amino acid or (NH(4))(2)SO(4) as ...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1964

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.87.6.1462-1470.1964